The predictor accepts protein sequences in FASTA format.

1. Type/paste your query peptide squences in the textfield to start the prediction.
2. Or you can upload the sequences in FASTA format.
3. Example sequences can be loaded by clicking on the respective link on top of the submission form.

We provide three optimized settings for typical scenarios and users can also provide a custom threshold.

1. Choose "High Specificity" if you want to limit the number of false positives and only list the most reliable predictions.
2. Choose "Best overall performance" if you want to have a good compromise between sensitivity and specificity.
3. Choose "High Sensitivity" if your query protein(s) are very likely to contain zinc-binding domains.
4. Provide custom threshold (bit score; a reasonable range would be between -10 and +20, higher values are more restrictive)

We combined the predicted zinc-binding motif from Hufzinc predictor with a curated list of PFAM zinc finger HMMs. We provide four settings for displaying different results.

1. Display all zinc binding motif(s) predicted by Hufzinc.
2. Display all zinc binding motif(s) ONLY predicted by Hufzinc.
3. Disply all zinc binding motif(s) predicted by both Hufzinc and PFAM zinc finger HMMs.
4. Display all zinc binding motif(s) ONLY predicted by PFAM zinc finger HMMS.

For each query sequence, we provide the following information according to the output option selected.

1. The description line of the sequence and its length
2. Display of all zinc binding motif(s) predicted by Hufzinc (one per line). This part will be displayed when output options 1, 2 or 3 are selected.
3. Display of all zinc binding motif(s) predicted by PFAM zinc finger HMMs (one per line). This part will be displayed when output options 3 or 4 are selected.
4. Display of summary information such as how many sequences were predicted from how many queries.
5. Download link for tab-seprated text output (especially useful for large number of sequences).