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Supplementary materials to the article: |
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Full reference
Nuclear import of a lipid-modified transcription factor: Mobilization of NFAT5 isoform a by osmotic stress
Cell Cycle Volume 10, Issue 22 November 15, 2011
Pages 3897 - 3911
http://dx.doi.org/10.4161/cc.10.22.18043
Birgit Eisenhaber, Michaela Sammer, Wai Heng Lua, Wolfgang Benetka, Lai Ling Liew, Weimiao Yu, Hwee Kuan Lee, Manfred Koranda, Frank Eisenhaber and Sharmila Adhikari
Supplementary Methods : Detailed description of experimental procedures
This file provides all methodical details for reproducing the experiments.
Filename : Supplement-Methods.pdf (~95 kb) download
Supplementary Material for Figure 1 : Detailed description of in silico screen, taxonomic overview and alignments
This file provides all methodical details for in silico part of this work.
Filename : Supplement-Figure-1.pdf (~638 kb) download
Supplementary Material for Figure 4 : Detailed description of image processing procedures
This file provides all methodical details for image processing.
Filename : Supplement-Figure-4.pdf (~87 kb) download
Supplement to Figure 3 : Full-size images for Figure 3
This file provides large images for the csubc ellular localization studies.
Filename : Full-Size-Figure-3.pdf (~3.2 Mb) download
Supplement to Figure 4 : Full-size images for Figure 4
This file provides representative large images for the translocation studies upon salt stress.
Filename : Full-Size-Figure-4.pdf (~4.6 Mb) download
Collections of fixed cell images used for Figure 4B
The images are provided in two formats. First, they can be downloaded as zip file of individual tiff images (attention: filesize ~130 MB!). We provide both the original tiffs and the segmented images. Second, they can be downloaded as one pdf file of all images (attention: file size ~25 MB).
- no DNA (zip, pdf)
- GFP control (zip, pdf)
- NFAT5c-GFP (zip, pdf)
- NFAT5b-GFP (zip, pdf)
- NFAT5a-GFP (zip, pdf)
- NFAT5a-G2A-GFP (zip, pdf)
- NFAT5a-2A-GFP (zip, pdf)
- myc-NFAT5a-GFP (zip, pdf)
- NFAT5a-C5A-GFP (zip, pdf)
- NFAT5a-C5_32A-GFP (zip, pdf)
- NFAT5a-C32_83_88_96A-GFP (zip, pdf)
Supplement to Figure 5 : Full-size images for Figure 5
This file provides large representative images for the imaging experiments exploring NFAT5a subcellular localization upon osmotic stress.
Filename : Full-Size-Figure-5.pdf (~3.7 Mb) download
Supplement to Figure 6 : Full-size images for Figure 6
This file provides large representative images for the live cell imaging experiments ("race" between NFAT5a/b/c into the nucleus upon osmotic stress).
Filename : Full-Size-Figure-6.pdf (~117 kb) download
Collections of live cell videos used for Figure 6B (attention: each video is ~80 MB!)
Supplement to Figure 7 : Full-size images for Figure 7
This file provides large images for the APMAP experiments.
Filename : Full-Size-Figure-7.pdf (~1.1 Mb) download
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