Mascot Search Results

User            : nedim
Email           : mujezinovic@imp.univie.ac.at
Search title    : Condensin Sample
MS data file    : Condensin.2423.2423.2.dta
Database        : nr  (3525863 sequences; 1211011241 residues)
Timestamp       : 26 Apr 2006 at 11:39:26 GMT
Significant hits: gi|2827693  unnamed protein product [Homo sapiens]

Probability Based Mowse Score

Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 57 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Score Distribution

Peptide Summary Report

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1.    gi|2827693                           Mass: 32438    Score: 58     Queries matched: 1
 unnamed protein product [Homo sapiens]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
1   430.41891   858.82327   858.45589   0.36739 0  58  0.045 1  R.QGEVLASR.K

 
      Proteins matching the same set of peptides:
      gi|12654905                          Mass: 60985    Score: 58     Queries matched: 1
 HCAP-H2 protein [Homo sapiens]
      gi|14495663                          Mass: 62954    Score: 58     Queries matched: 1
 Kleisin beta [Homo sapiens]
      gi|23097285                          Mass: 62823    Score: 58     Queries matched: 1
 kleisin beta isoform 2 [Homo sapiens]
      gi|23308757                          Mass: 27209    Score: 58     Queries matched: 1
 kleisin beta isoform 1 [Homo sapiens]
      gi|47678739                          Mass: 68640    Score: 58     Queries matched: 1
 Em:U62317.2 [Homo sapiens]
      gi|48257119                          Mass: 60208    Score: 58     Queries matched: 1
 HCAP-H2 protein [Homo sapiens]

2.    gi|51950222                          Mass: 71779    Score: 54     Queries matched: 1
 MGC81656 protein [Xenopus laevis]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.49226   0.33101 1  58  0.045 1  R.KGEVLASR.K

 
      Proteins matching the same set of peptides:
      gi|89273751                          Mass: 71563    Score: 54     Queries matched: 1
 novel protein [Xenopus tropicalis]
3.    gi|32445567                          Mass: 67847    Score: 44     Queries matched: 1
 conserved hypothetical protein [Rhodopirellula baltica SH 1]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.45589   0.36739 0  44  1.2 3  R.GQEVLASR.N

4.    gi|1175815                           Mass: 26708    Score: 39     Queries matched: 1
 Putative HTH-type transcriptional regulator HI1476
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.41951   0.40376 1  39  4.1 5  R.KGEADPSR.S

 
      Proteins matching the same set of peptides:
      gi|75429032                          Mass: 27031    Score: 39     Queries matched: 1
 conserved hypothetical protein HI1476 [Actinobacillus succinogenes 130Z]
5.    gi|50742688                          Mass: 248130   Score: 39     Queries matched: 1
 PREDICTED: similar to KIAA1244 [Gallus gallus]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.49226   0.33101 1  39  4.1 5  K.KGELLGSR.G

6.    gi|89295598                          Mass: 300482   Score: 37     Queries matched: 1
 cation channel family protein [Tetrahymena thermophila SB210]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   857.50825   1.31502 1  39  3.6 4  K.KGQVIASR.Y

7.    gi|37533160                          Mass: 24076    Score: 37     Queries matched: 1
 hypothetical protein [Oryza sativa (japonica cultivar-group)]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.49226   0.33101 1  37  6.8 7  K.KGEVTGIR.G

8.    gi|50908125                          Mass: 56907    Score: 37     Queries matched: 1
 fertility restorer homologue A-like [Oryza sativa (japonica cultivar-group)]
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      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.45587   0.36740 1  37  6.8 7  K.KGEVEAAR.G

9.    gi|83575977                          Mass: 75607    Score: 34     Queries matched: 1
 ATP-dependent DNA helicase RecG [Rhodospirillum rubrum ATCC 11170]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.45587   0.36740 0  36  7.7 9  R.GAGEVLGTR.Q

10.   gi|76609376                          Score: 25     Queries matched: 1
 PREDICTED: similar to titin isoform N2-B [Bos taurus]
Check to include this hit in error tolerant search or archive report
       
      Query  Observed  Mr(expt)  Mr(calc)   Delta Miss Score Expect Rank Peptide
 1   430.41891   858.82327   858.49228   0.33099 1  35  10 10  K.KGIDLASR.A

Search Parameters

Type of search         : MS/MS Ion Search
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages   : 1
Instrument type        : Default
Number of queries      : 1

Mascot:  http://www.matrixscience.com/

Top scoring peptide matches to query 1
Score greater than 57 indicates identity
Status bar shows all hits for this peptide
Score   Delta  Hit  Protein     Peptide
 58.3    0.33    2  gi|51950222 R.KGEVLASR.K
 58.3    0.37    1  gi|2827693  R.QGEVLASR.K
 44.0    0.37    3  gi|32445567 R.GQEVLASR.N
 39.4    1.32    6  gi|89295598 K.KGQVIASR.Y
 38.8    0.40    4  gi|1175815  KGEADPSR
 38.8    0.33    5  gi|50742688 K.KGELLGSR.G
 36.5    0.37    8  gi|50908125 K.KGEVEAAR.G
 36.5    0.33    7  gi|37533160 K.KGEVTGIR.G
 36.0    0.37    9  gi|83575977 R.GAGEVLGTR.Q
 34.8    0.33   10  gi|76609376 K.KGIDLASR.A